Hello. Thank you for visiting our store, we buy things in a licensed pharmacy in the European Union. This test detects the acrosomal protein SP-10 in semen. The assay is based on the binding of SP-10 (if present) to conjugated anti-SP-10 antibodies in the sample. A sperm concentration of ? 15 million/mL is the internationally accepted minimum sperm count for a fertile man. Composition Test cassettes (2 pcs.), collection cups (2 pcs.), seed transfer medium, sample dilution buffer, workbench, instructions for use. Dose The sample should be collected within 3-7 days after the last ejaculation. In case of masturbation, the semen should be collected directly into the semen collection cup. Do not touch the semen sample with your hands, tissues or other materials, as this may contaminate it. Shake the seed sample jar thoroughly, set aside and leave at room temperature for 1 hour until the seeds liquefy. 1. Using the seed transfer tool, remove the required amount of sample (follow the 0.1 ml mark). 2. Pour the seed sample into the enclosed bottle of sample dilution buffer and close tightly with the pipette and cap. 3. Swirl the sample up and down several times to mix (recommended up to 10 times). 4. Use the test cartridge in the sealed foil pouch to test the sample. Holding the cartridge on a flat surface, apply two drops to the sample testing area (S). 5. After 5 minutes, read the result. Read the results using the leaflet. Handle all used kit components according to local requirements. If possible, bag biohazardous waste.